Topoisomerase I anticorps

N° du produit ABIN6945193

L’anticorps Lapin Monoclonal anti-Topoisomerase I a été validé pour WB, IHC, FACS, IHC (p) et IF (cc). Il convient pour détecter Topoisomerase I dans des échantillons de Humain et Souris.

Aperçu rapide pour Topoisomerase I anticorps (ABIN6945193)

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase I est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 1D1

Détail du produit anti-Topoisomerase I anticorps

Réactivité croisée: Humain, Souris

Purification: Purified by Protein A.

Immunogène: Human Topoisomerase I between 1-100 amino acids

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur Topoisomerase I

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Autre désignation: Topoisomerase I

Sujet:

Synonyms: DNA topoisomerase 1, DNA topoisomerase I, TOP1

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

ID gène: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance