Histone 3 anticorps (H3K27me3)

N° du produit ABIN6952338

L’anticorps Lapin Polyclonal anti-Histone 3 a été validé pour WB, IF, ELISA, ChIP, DB, ChIP-seq et CUT&Tag. Il convient pour détecter Histone 3 dans des échantillons de Humain.

Aperçu rapide pour Histone 3 anticorps (H3K27me3) (ABIN6952338)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Classe de qualité: ChIP-seq Grade

Détail du produit anti-Histone 3 anticorps

Épitope: H3K27me3

Specificité: Polyclonal antibody raised in rabbit against against histone H3, trimethylated at lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide.

Réactivité croisée: Arabidopsis, Boeuf (Vache), Mouche des fruits (Drosophila melanogaster), Souris, Porc, Rat (Rattus), Schistosoma japonicum, Poisson zèbre (Danio rerio)

Purification: Peptide affinity purified

Immunogène: synthetic peptide

Information d'application

Indications d'application: ChIP/ChIP-seq 1 μg:ChIP
ELISA 1:5,000
Dot Blot 1:20,000
WB 1:1,000
IF 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.6 μg/μL

Buffer: PBS, 0.05 % azide, 0.05 % ProClin300

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone 3

Sujet: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of histone H3K27 is associated with gene repression.

UniProt: P68431