Histone 3 anticorps (H3K4me3)

N° du produit ABIN6952351

Cet anticorps anti-Histone 3 est un anticorps Lapin Polyclonal détectant Histone 3 dans WB, IF, ELISA, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag, PeA et SNAP-ChIP. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour Histone 3 anticorps (H3K4me3) (ABIN6952351)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA), Sample Normalization and Antibody Profiling ChIP (SNAP-ChIP)

Classe de qualité: ChIP-seq Grade

Détail du produit anti-Histone 3 anticorps

Épitope: H3K4me3

Specificité: Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 4 (H3K4me3), using a KLH-conjugated synthetic peptide.

Réactivité croisée: Arabidopsis, Daphnia, Maize/Corn (Zea mays), Souris, Poplar (Populus), Riz (Oryza sativa), Tomate (Solanum lycopersicum), Truite, Poisson zèbre (Danio rerio)

Réactivité croisée (Details): wide range

Purification: Peptide affinity purified

Immunogène: synthetic peptide

Information d'application

Indications d'application: ChIP/ChIP-seq<1 μg:ChIP
ELISA 1:1,000
Dot Blot 1:10,000
Peptde Array 1:10,000
WB 1:1,000
IF 1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.3 μg/μL

Buffer: PBS, 0.05 % azide, 0.05 % ProClin300

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-Histone 3 (ABIN6952351)

Schreiber, Mercier, Jiménez, Ye, García-Sánchez, Klein, Meunier, Ghimire, Birck, Jost, de Lichtenberg, Honoré, Serup, Gradwohl: "Extensive NEUROG3 occupancy in the human pancreatic endocrine gene regulatory network." dans: Molecular metabolism, Vol. 53, pp. 101313, (2021) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone 3

Sujet: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K4 is associated with activation of gene transcription.

UniProt: P68431