Recombinant Blue Fluorescent Protein anticorps (AZDye 568)

N° du produit ABIN6953245

L’anticorps Alpaca Monoclonal anti-Blue Fluorescent Protein a été validé pour FACS, ICC et IF. Il convient pour détecter Blue Fluorescent Protein dans des échantillons de Entacmaea quadricolor.

Aperçu rapide pour Recombinant Blue Fluorescent Protein anticorps (AZDye 568) (ABIN6953245)

Antigène: Blue Fluorescent Protein (BFP)

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Entacmaea quadricolor

Hôte: Alpaca

Clonalité: Monoclonal

Conjugué: Cet anticorp Blue Fluorescent Protein est conjugé à/à la AZDye 568

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1H7

Détail du produit anti-Blue Fluorescent Protein anticorps (AZDye 568)

Expression System: E.coli

Fonction: Camelid sdAb anti-TagBFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 1H7

Specificité: Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor

Réactivité croisée (Details): Does not cross-react with common GFP- or dsRed derivatives.

Attributs du produit: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Purification: Produced in: E.coli

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Information d'application

Indications d'application: Recommended dilution 1:500

Commentaires:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Concentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Conseil sur la manipulation: Protect from light!

Stock: -20 °C,-80 °C

Stockage commentaire: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Date de péremption: 12 months

Détails sur Blue Fluorescent Protein

Antigène: Blue Fluorescent Protein (BFP)

Autre désignation: TagBFP