CD8 alpha anticorps

N° du produit ABIN6961722

L’anticorps Souris Monoclonal anti-CD8 alpha a été validé pour FACS, IP et IHC (fro). Il convient pour détecter CD8 alpha dans des échantillons de Humain.

Aperçu rapide pour CD8 alpha anticorps (ABIN6961722)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RPA-T8

Détail du produit anti-CD8 alpha anticorps

Réactivité croisée: Babouin, Chimpanzé, Cynomolgus, Macaque rhésus, Sooty mangabey (Cercocebus atys)

Purification: This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze.

Isotype: IgG1 kappa

Information d'application

Indications d'application: This purified format is guaranteed to be >90 % pure as determined by SDS-PAGE analysis.

Commentaires:

0.5 mg/mL

Restrictions: For Research Use only

Stockage

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: 2-8°C

Date de péremption: 12 months

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8a

Sujet: The RPA-T8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a ho- modimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone Hit8a, the RPA-T8 antibody will not block binding of Hit8a to CD8a.

ID gène: 925

UniProt: P01732

Pathways: TCR Signaling