CD8 alpha anticorps (redFluor™ 710)

N° du produit ABIN7278584

L’anticorps Souris Monoclonal anti-CD8 alpha a été validé pour FACS. Il convient pour détecter CD8 alpha dans des échantillons de Humain.

Aperçu rapide pour CD8 alpha anticorps (redFluor™ 710) (ABIN7278584)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est conjugé à/à la redFluor™ 710

Application: Flow Cytometry (FACS)

Clone: RPA-T8

Détail du produit anti-CD8 alpha anticorps (redFluor™ 710)

Réactivité croisée: Babouin, Chimpanzé, Cynomolgus, Macaque rhésus, Sooty mangabey (Cercocebus atys)

Purification: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.

Isotype: IgG1 kappa

Information d'application

Indications d'application: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. redFluor™ 710 dye is excited by the red (633-647 nm) laser and has a peak emission of 710 nm. The recommended band pass filter for this dye is 710/50. redFluor™ 710 can be used as an alternative for Alexa Fluor® 700. Confirm that your cytometer is configured to detect this fluorochrome.

Commentaires:

5 uL (0.125 ug)/test

Restrictions: For Research Use only

Stockage

Buffer: 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: 2-8°C protected from light

Date de péremption: 12 months

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8a

Sujet: The RPA-T8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, Hit8a, the RPA-T8 antibody will not block binding of Hit8a to CD8a.

ID gène: 925

UniProt: P01732

Pathways: TCR Signaling