IL-2 anticorps (PromoFluor 647 Premium)

N° du produit ABIN6963756

L’anticorps Souris Monoclonal anti-IL-2 a été validé pour FACS. Il convient pour détecter IL-2 dans des échantillons de Boeuf (Vache) et Mouton.

Aperçu rapide pour IL-2 anticorps (PromoFluor 647 Premium) (ABIN6963756)

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivité: Boeuf (Vache), Mouton

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-2 est conjugé à/à la PromoFluor 647 Premium

Application: Flow Cytometry (FACS)

Clone: MT3B3

Détail du produit anti-IL-2 anticorps (PromoFluor 647 Premium)

Specificité: Native and recombinant IL-2

Réactivité croisée (Details): The monoclonal antibody cross-reacts with IL-2 from sheep. For dection of IL-2 in sheep, unconjugated MT3B3 with second step detection is recommended.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogène: Recombinant bovine IL-2

Isotype: IgG1

Information d'application

Indications d'application: Detection of IL-2 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-2 by flow cytometry in viable bovine PBMC. Cells were stimulated for 5 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were then fixed and permeabilized using 4 % paraformaldehyde and saponin, and subsequently stained with anti- bovine IL-2 mAb MT3B3-PF647P (solid line). Matched isotype control antibody MT128. The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Date de péremption: 18 months

Détails sur IL-2

Antigène: IL-2 (IL2) (Interleukin 2 (IL2))

Autre désignation: IL2

ID gène: 280822

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation