IL-4 anticorps (PromoFluor-488 Premium)

N° du produit ABIN6963827

Cet anticorps Rat Monoclonal détecte spécifiquement IL-4 dans FACS. Il présente une réactivité envers Humain et Non-Human Primate.

Aperçu rapide pour IL-4 anticorps (PromoFluor-488 Premium) (ABIN6963827)

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité: Humain, Non-Human Primate

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-4 est conjugé à/à la PromoFluor-488 Premium

Application: Flow Cytometry (FACS)

Clone: IL4-3

Détail du produit anti-IL-4 anticorps (PromoFluor-488 Premium)

Specificité: Native IL-4

Réactivité croisée (Details): The monoclonal antibody cross-reacts with IL-4 from non-human primates.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogène: Recombinant human IL-4

Isotype: IgG1

Information d'application

Indications d'application: Detection of IL-4 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-4 by flow cytometry in viable human PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were stained with anti-CD3-BV421, fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with PF488P-conjugated mAb IL4-3 (right panel). Non-stimulated cells shown as reference (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: in PBS with 0.2 % BSA and 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Date de péremption: 18 months

Détails sur IL-4

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: IL4

ID gène: 3565

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation