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IL-4 anticorps (PromoFluor 647 Premium)

IL4 Reactivité: Humain, Non-Human Primate FACS Hôte: Rat Monoclonal IL4-3 PromoFluor 647 Premium
N° du produit ABIN6963828
  • Antigène Voir toutes IL-4 (IL4) Anticorps
    IL-4 (IL4) (Interleukin 4 (IL4))
    Reactivité
    • 145
    • 67
    • 24
    • 14
    • 11
    • 10
    • 10
    • 8
    • 7
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Humain, Non-Human Primate
    Hôte
    • 109
    • 74
    • 63
    • 7
    • 3
    • 2
    Rat
    Clonalité
    • 148
    • 107
    • 1
    Monoclonal
    Conjugué
    • 150
    • 33
    • 17
    • 14
    • 10
    • 8
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Cet anticorp IL-4 est conjugé à/à la PromoFluor 647 Premium
    Application
    • 123
    • 117
    • 72
    • 72
    • 25
    • 24
    • 21
    • 18
    • 13
    • 13
    • 12
    • 11
    • 9
    • 9
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificité
    Native IL-4
    Réactivité croisée (Details)
    The monoclonal antibody cross-reacts with IL-4 from non-human primates.
    Purification
    Purified from in vitro cultures by protein G affinity chromatography.
    Immunogène
    Recombinant human IL-4
    Clone
    IL4-3
    Isotype
    IgG1
    Top Product
    Discover our top product IL4 Anticorps primaire
  • Indications d'application
    Detection of IL-4 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-4 by flow cytometry in viable human PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were stained with anti-CD3-BV421, fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with PF647P-conjugated mAb IL4-3 (right panel). Non-stimulated cells shown as reference (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    in PBS with 0.2 % BSA and 0.09 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
    Date de péremption
    18 months
  • Antigène
    IL-4 (IL4) (Interleukin 4 (IL4))
    Autre désignation
    IL4 (IL4 Produits)
    Synonymes
    anticorps IL4, anticorps IL-4, anticorps BCGF-1, anticorps BCGF1, anticorps BSF-1, anticorps BSF1, anticorps Il-4, anticorps Il4e12, anticorps IL2, anticorps interleukin 4, anticorps IL4, anticorps Il4
    ID gène
    3565
    Pathways
    Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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