For quantification of apoE in serum/plasma samples and cell culture supernatants using ELISA and for enumeration of apoE producing cells using ELISpot. E276 is recommended as coating mAb in combination with detection mAb E887 . The antibody is also suitable for immunoprecipitation and Western blot.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.
Date de péremption
18 months
van Harten, Jongbloed, Teunissen, Scheltens, Veerhuis, van der Flier: "CSF ApoE predicts clinical progression in nondemented APOEε4 carriers." dans: Neurobiology of aging, Vol. 57, pp. 186-194, (2018) (PubMed).
Bissig-Choisat, Wang, Legras, Saha, Chen, Bell, Pankowicz, Hill, Barzi, Leyton, Leung, Kruse, Himes, Goss, Wilson, Chan, Lagor, Bissig: "Development and rescue of human familial hypercholesterolaemia in a xenograft mouse model." dans: Nature communications, Vol. 6, pp. 7339, (2015) (PubMed).
Braesch-Andersen, Paulie, Smedman, Mia, Kumagai-Braesch: "ApoE production in human monocytes and its regulation by inflammatory cytokines." dans: PLoS ONE, Vol. 8, Issue 11, pp. e79908, (2014) (PubMed).
Handattu, Nayyar, Garber, Palgunachari, Monroe, Keenum, Mishra, Datta, Anantharamaiah: "Two apolipoprotein E mimetic peptides with similar cholesterol reducing properties exhibit differential atheroprotective effects in LDL-R null mice." dans: Atherosclerosis, Vol. 227, Issue 1, pp. 58-64, (2013) (PubMed).
Liu, Kuhel, Shen, Hui, Woods: "Apolipoprotein E does not cross the blood-cerebrospinal fluid barrier, as revealed by an improved technique for sampling CSF from mice." dans: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 303, Issue 9, pp. R903-8, (2013) (PubMed).