APOE anticorps (Biotin)

N° du produit ABIN6963938

Cet anticorps Souris Monoclonal détecte spécifiquement APOE dans WB, ELISA et ELISpot. Il présente une réactivité envers Humain et Non-Human Primate et a été mentionné dans 5+ publications.

Aperçu rapide pour APOE anticorps (Biotin) (ABIN6963938)

Antigène: APOE (Apolipoprotein E (APOE))

Reactivité: Humain, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp APOE est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, ELISpot

Clone: E887

Détail du produit anti-APOE anticorps (Biotin)

Specificité: Native and recombinant apolipoprotein E (isoforms apoE2, apoE3 and apoE4).

Réactivité croisée (Details): The monoclonal antibody cross-reacts with apoE from non-human primates.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogène: Purified human apolipoprotein E

Isotype: IgG1

Information d'application

Indications d'application: For quantification of apoE in serum/plasma samples and cell culture supernatants using ELISA and for enumeration of human apoE producing cells using ELISpot. E887 is recommended as detection mAb in combination with coating mAb E276 . The antibody is also suitable for immunoprecipitation and Western blot.

Commentaires:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing.

Date de péremption: 18 months

Références pour anticorps anti-APOE (Biotin) (ABIN6963938)

Bissig-Choisat, Wang, Legras, Saha, Chen, Bell, Pankowicz, Hill, Barzi, Leyton, Leung, Kruse, Himes, Goss, Wilson, Chan, Lagor, Bissig: "Development and rescue of human familial hypercholesterolaemia in a xenograft mouse model." dans: Nature communications, Vol. 6, pp. 7339, (2015) (PubMed).

Bankwitz, Vieyres, Hueging, Bitzegeio, Doepke, Chhatwal, Haid, Catanese, Zeisel, Nicosia, Baumert, Kaderali, Pietschmann: "Role of hypervariable region 1 for the interplay of hepatitis C virus with entry factors and lipoproteins." dans: Journal of virology, Vol. 88, Issue 21, pp. 12644-55, (2014) (PubMed).

Braesch-Andersen, Paulie, Smedman, Mia, Kumagai-Braesch: "ApoE production in human monocytes and its regulation by inflammatory cytokines." dans: PLoS ONE, Vol. 8, Issue 11, pp. e79908, (2014) (PubMed).

Handattu, Nayyar, Garber, Palgunachari, Monroe, Keenum, Mishra, Datta, Anantharamaiah: "Two apolipoprotein E mimetic peptides with similar cholesterol reducing properties exhibit differential atheroprotective effects in LDL-R null mice." dans: Atherosclerosis, Vol. 227, Issue 1, pp. 58-64, (2013) (PubMed).

Liu, Kuhel, Shen, Hui, Woods: "Apolipoprotein E does not cross the blood-cerebrospinal fluid barrier, as revealed by an improved technique for sampling CSF from mice." dans: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 303, Issue 9, pp. R903-8, (2013) (PubMed).

Détails sur APOE

Antigène: APOE (Apolipoprotein E (APOE))

Autre désignation: apoE

ID gène: 348

Pathways: Regulation of Cell Size, Lipid Metabolism