BUB1 anticorps (AA 281-419)

N° du produit ABIN6971474

Cet anticorps Souris Monoclonal détecte spécifiquement BUB1 dans IF et ICC. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour BUB1 anticorps (AA 281-419) (ABIN6971474)

Antigène: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BUB1 est non-conjugé

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 14H5

Détail du produit anti-BUB1 anticorps

Épitope: AA 281-419

Attributs du produit: Bub1 is a serine-threonine protein kinase and an important regulator of cell division. It is essential for the G2/M mitotic spindle assembly/integrity checkpoint as well as critical for chromosome alignment. Bub1 is required for the assembly of a large number of proteins at the kinetochore, including CENP-E, CENP-F, BUB1B, MAD2L1 and PLK1. It plays a role in sister chromatid cohesion and it serves as a substrate for the anaphase-promoting complex. Bub1 mediates cell death in response to chromosome mis-segregation and acts to suppress spontaneous tumorigenesis. Bub1 antibody (mAb) (Clone 14H5) was raised in a Mouse host. It has been validated for use in Immunocytochemistry and Immunofluorescence, it has been shown to react with Human and Mouse samples.

Purification: Protein G Chromatography

Immunogène: This Bub1 antibody was raised against a recombinant protein corresponding to amino acids 281-419 of human Bub1.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur BUB1

Antigène: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Autre désignation: Bub1

Poids moléculaire: 130 kDa

NCBI Accession: NP_004327