POLR2F anticorps

N° du produit ABIN6972612

L’anticorps Rat Monoclonal anti-POLR2F a été validé pour WB, IF, ChIP, ChIP-seq et ICC. Il convient pour détecter POLR2F dans des échantillons de Humain.

Aperçu rapide pour POLR2F anticorps (ABIN6972612)

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2F est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunocytochemistry (ICC)

Clone: 1F4B6

Détail du produit anti-POLR2F anticorps

Attributs du produit: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. RNA pol II antibody (mAb) (Clone 1F4B6) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a synthetic peptide containing the CTD of human RNA Polymerase II.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur POLR2F

Antigène: POLR2F (Polymerase (RNA) II (DNA Directed) Polypeptide F (POLR2F))

Autre désignation: RNA pol II

Poids moléculaire: 240 kDa

NCBI Accession: NP_000928

Pathways: Regulatory RNA Pathways