Rpb1 CTD anticorps (pSer5)

N° du produit ABIN6972638

Cet anticorps Rat Monoclonal détecte spécifiquement Rpb1 CTD dans WB, IF et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour Rpb1 CTD anticorps (pSer5) (ABIN6972638)

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Rpb1 CTD est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1H4B6

Détail du produit anti-Rpb1 CTD anticorps

Épitope: pSer5

Attributs du produit: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription. RNA pol II CTD phospho Ser5 antibody (mAb) (Clone 1H4B6) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur Rpb1 CTD

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Autre désignation: RNA pol II CTD

Poids moléculaire: 240 kDa

NCBI Accession: NP_000928