TET1 anticorps (C-Term)

N° du produit ABIN6972860

Cet anticorps Lapin Polyclonal détecte spécifiquement TET1 dans WB, IHC, ChIP et ChIP-seq. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour TET1 anticorps (C-Term) (ABIN6972860)

Antigène: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TET1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Détail du produit anti-TET1 anticorps

Épitope: C-Term

Attributs du produit: Tet1 (Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Tet1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunohistochemistry and Western blot, it has been shown to react with Human and Mouse samples.

Purification: Affinity Purified

Immunogène: This antibody was raised against a peptide within the C-terminal region of mouse Tet1.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur TET1

Antigène: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Autre désignation: Tet1

Poids moléculaire: 215 kDa

NCBI Accession: NP_081660

Pathways: Stem Cell Maintenance, L'effet Warburg