NOS2 anticorps (AA 1051-1153)

N° du produit ABIN6989274

Cet anticorps anti-NOS2 est un anticorps Lapin Polyclonal détectant NOS2 dans WB, ICC, FACS, IHC (p), IF (p), IF (cc) et IHC (fro). Adapté pour Humain, Souris et Rat.

Aperçu rapide pour NOS2 anticorps (AA 1051-1153) (ABIN6989274)

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOS2 est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-NOS2 anticorps

Épitope: AA 1051-1153

Réactivité croisée: Humain, Souris, Rat

Homologie: Dog,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human iNos/Nos-2

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur NOS2

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Autre désignation: iNos/Nos-2,

Sujet:

Synonyms: i NOS, Nitric Oxide Synthase, Inducible, HEP NOS, Hepatocyte NOS, HEPNOS, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, INOS,I-NOS, Inosl, MAC NOS, Macrophage NOS, Nitric oxide synthase 2 inducible macrophage, Nitric oxide synthase 2A (inducible hepatocytes), Nitric oxide synthase inducible, NOS 2, NOS 2A, NOS, Nos II, NOS type II, Nos2, NOS2A, NOS2_HUMAN.

Background: Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

ID gène: 4843

UniProt: P35228

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process