GSK3 alpha/beta anticorps (pTyr216, pTyr279)

N° du produit ABIN6989926

Cet anticorps Lapin Monoclonal détecte spécifiquement GSK3 alpha/beta dans WB, IHC, IHC (p), IF (cc) et IP. Il présente une réactivité avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour GSK3 alpha/beta anticorps (pTyr216, pTyr279) (ABIN6989926)

Antigène: GSK3 alpha/beta (GSK3a/b) (Glycogen Synthase Kinase 3 alpha/beta (GSK3a/b))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp GSK3 alpha/beta est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunoprecipitation (IP)

Clone: 5G8

Détail du produit anti-GSK3 alpha/beta anticorps

Épitope: pTyr216, pTyr279

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: Synthetic phospho-Peptide corresponding to residues surrounding Tyr216 and 279 of human GSK3(alpha+beta).

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IP(1-2 μg)
IHC()

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur GSK3 alpha/beta

Antigène: GSK3 alpha/beta (GSK3a/b) (Glycogen Synthase Kinase 3 alpha/beta (GSK3a/b))

Autre désignation: GSK3(alpha+beta)

Sujet:

Synonyms: Factor A antibody, Glycogen synthase kinase 3 alpha antibody, Glycogen synthase kinase 3 beta antibody, GSK3 alpha antibody, GSK3 beta antibody, GSK3B antibody

Background: Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

ID gène: 2932

UniProt: P49840