DFFA anticorps (C-Term)

N° du produit ABIN6990254

L’anticorps Lapin Polyclonal anti-DFFA a été validé pour WB, ELISA, IF et ICC. Il convient pour détecter DFFA dans des échantillons de Humain.

Aperçu rapide pour DFFA anticorps (C-Term) (ABIN6990254)

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DFFA est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-DFFA anticorps

Épitope: C-Term

Purification: DFF45 Antibody is Antibody is DEAE purified.

Immunogène: DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45. The immunogen is located within the last 50 amino acids of DFF45.

Isotype: IgG

Information d'application

Indications d'application: DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μ,g/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: DFF45 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: DFF45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur DFFA

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Autre désignation: DFF45

Sujet: DFF45 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.

Poids moléculaire: 45 kDa

ID gène: 1676

NCBI Accession: NP_004392

UniProt: O00273

Pathways: Apoptose, Caspase Cascade in Apoptosis