FLASH anticorps (C-Term)

N° du produit ABIN6990336

Détail du produit anti-FLASH anticorps

Antigène: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FLASH est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Purification: FLASH Antibody is affinity chromatography purified via peptide column.

Immunogène: FLASH antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human FLASH which differ from those of mouse by one amino acid. The immunogen is located within the last 50 amino acids of FLASH.

Isotype: IgG

Information d'application

Indications d'application: FLASH antibody can be used for detection of FLASH by Western blot at 0.5 μ,g/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: FLASH Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: FLASH antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur FLASH

Antigène: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Autre désignation: FLASH (CASP8AP2 Produits)

Synonymes: anticorps CASP8AP2, anticorps FLASH, anticorps fc74h10, anticorps wu:fc74h10, anticorps zgc:113449, anticorps CED-4, anticorps RIP25, anticorps AA387232, anticorps D4Ertd659e, anticorps mKIAA1315, anticorps caspase 8 associated protein 2, anticorps CASP8AP2, anticorps casp8ap2, anticorps Casp8ap2

Sujet: FLASH Antibody: A novel mammalian CED-4 homologous was recently identified and cloned in mouse and human and designated FLASH (for FLICE-associated huge protein). FLASH is involved in Fas induced apoptosis. It is recruited to Fas after the receptor cross-linking. Overexpression of wild type of FLASH facilitates and its dominant negative form inhibits Fas induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH and mediates activation of caspase-8. There are parallels between FLASH and Apaf-1/CED-4 although there are arguments against their structural similarity. FLASH is widely expressed.

Poids moléculaire: 220 kDa

ID gène: 9994

UniProt: Q9UKL3

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity