Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
Purification
TOLLIP Antibody is affinity chromatography purified via peptide column.
Immunogène
TOLLIP antibody was raised against a 16 amino acid synthetic peptide from near the center of human TOLLIP. The immunogen is located within amino acids 70 - 120 of TOLLIP.
TOLLIP
Reactivité: Humain, Souris
WB, IP
Hôte: Rat
Monoclonal
Kimmy-1
unconjugated
Indications d'application
TOLLIP antibody can be used for the detection of TOLLIP by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL.
Antibody validated: Western Blot in rat samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
TOLLIP Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
TOLLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
TOLLIP Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include TOLLIP, MyD88, and TRIF. TOLLIP associates directly with TLR2 and TLR 4, acting as an inhibitor to TLR activation. This negative regulation of TLR signaling may serve to limit the production of proinflammatory mediators during infection and inflammation.