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beta Actin anticorps (C-Term)

Cet anticorps Lapin Polyclonal détecte spécifiquement beta Actin dans WB, ELISA, IF et ICC. Il présente une réactivité envers Humain, Souris et Rat.
N° du produit ABIN6990625

Aperçu rapide pour beta Actin anticorps (C-Term) (ABIN6990625)

Antigène

Voir toutes beta Actin (ACTB) Anticorps
beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité

  • 180
  • 135
  • 115
  • 54
  • 43
  • 43
  • 38
  • 26
  • 26
  • 24
  • 17
  • 14
  • 11
  • 10
  • 7
  • 7
  • 6
  • 5
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Souris, Rat

Hôte

  • 111
  • 88
  • 5
  • 1
  • 1
Lapin

Clonalité

  • 107
  • 97
Polyclonal

Conjugué

  • 137
  • 13
  • 12
  • 9
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp beta Actin est non-conjugé

Application

  • 171
  • 75
  • 62
  • 42
  • 31
  • 27
  • 26
  • 25
  • 13
  • 13
  • 7
  • 6
  • 6
  • 6
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Épitope

    • 41
    • 16
    • 14
    • 8
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Purification

    Beta-actin Antibody is affinity chromatography purified via peptide column.

    Immunogène

    Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beta-actin. The immunogen is located within the last 50 amino acids of Beta-actin.

    Isotype

    IgG
  • Indications d'application

    Beta-actin antibody can be used for the detection of Beta-actin by Western blot at 0.5 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

    Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Beta-actin Antibody is supplied in PBS containing 0.02 % sodium azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    -20 °C,4 °C

    Stockage commentaire

    Beta-actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
  • Antigène

    beta Actin (ACTB) (Actin, beta (ACTB))

    Autre désignation

    Beta-actin

    Sujet

    Beta-actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

    Poids moléculaire

    Predicted: 41 kDa

    Observed: 41 kDa

    ID gène

    60

    UniProt

    P60709

    Pathways

    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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