KEAP1 anticorps (C-Term)

N° du produit ABIN6990770

L’anticorps Lapin Polyclonal anti-KEAP1 a été validé pour WB, ELISA et IF. Il convient pour détecter KEAP1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour KEAP1 anticorps (C-Term) (ABIN6990770)

Antigène: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KEAP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-KEAP1 anticorps

Épitope: C-Term

Specificité: At least two isoforms of KEAP1 are known to exist.

Purification: KEAP1 Antibody is affinity chromatography purified via peptide column.

Immunogène: Anti-KEAP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human KEAP1. The immunogen is located within the last 50 amino acids of KEAP1.

Isotype: IgG

Information d'application

Indications d'application: WB: 1-2 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: KEAP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: KEAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur KEAP1

Antigène: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Autre désignation: KEAP1

Sujet: KEAP1 Antibody: KEAP1 (kelch-like ECH-associated protein 1) is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates NRF2 (NF-E2-related factor 2) and plays a role in the oxidative stress response. It targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome. KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain which are required for interaction with NRF2, and in binding Cul3-E3 ubiquitin ligase. Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD). KEAP1 also targets the down regulation of NF-κ,B activity by targeting IKKβ, degradation. Mutation of the KEAP1 gene is found in lung cancer.

Poids moléculaire: Predicted: 70kD

Observed: 65-70kD kDa

ID gène: 9817

NCBI Accession: NP_987096

UniProt: Q14145

Pathways: Maintenance of Protein Location