ATG5 anticorps (N-Term)

N° du produit ABIN6991065

Cet anticorps anti-ATG5 est un anticorps Poulet Polyclonal détectant ATG5 dans WB, ELISA et IHC (p). Adapté pour Humain et Souris.

Aperçu rapide pour ATG5 anticorps (N-Term) (ABIN6991065)

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivité: Humain, Souris

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG5 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ATG5 anticorps

Épitope: N-Term

Specificité: Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.

Purification: ATG5 Antibody is affinity chromatography purified via peptide column.

Immunogène: Anti-ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5. The immunogen is located within the first 50 amino acids of ATG5.

Isotype: IgY

Information d'application

Indications d'application: WB: 1-2 μ,g/mL, IHC-P: 2.5 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: ATG5 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: ATG5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur ATG5

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Autre désignation: ATG5

Sujet: ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.

Poids moléculaire: Predicted: 32kD

Observed: 55kD (ATG5-ATG12 conjugation) kDa

ID gène: 9474

UniProt: A9UGY9

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy