GLS2 anticorps (AA 300-350)

N° du produit ABIN6991498

Détail du produit anti-GLS2 anticorps

Antigène: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Épitope: AA 300-350

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLS2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificité: Multiple isoforms of GLS2 are known to exist.

Purification: GLS2 Antibody is affinity chromatography purified via peptide column.

Immunogène: GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2. The immunogen is located within amino acids 300 - 350 of GLS2.

Isotype: IgG

Information d'application

Indications d'application: GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: GLS2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: GLS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur GLS2

Antigène: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Autre désignation: GLS2 (GLS2 Produits)

Synonymes: anticorps si:ch211-216k22.10, anticorps GA, anticorps GLS, anticorps LGA, anticorps hLGA, anticorps A330074B06Rik, anticorps AI195532, anticorps Lga, anticorps Ga, anticorps glutaminase 2, anticorps glutaminase 2a (liver, mitochondrial), anticorps glutaminase 2 (liver, mitochondrial), anticorps glsA2, anticorps LOAG_03311, anticorps gls2a, anticorps GLS2, anticorps Gls2

Sujet: GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.

Poids moléculaire: Predicted: 36, 55, 62, 66 kDa

Observed: 55, 62 kDa

ID gène: 27165

NCBI Accession: NP_037399

UniProt: Q9UI32

Pathways: Dicarboxylic Acid Transport, L'effet Warburg