L’anticorps Lapin Polyclonal anti-BHLHA15 a été validé pour ELISA, WB, IF et IHC (p). Il convient pour détecter BHLHA15 dans des échantillons de Humain, Souris et Rat.
BHLHA15 antibody is human, mouse and rat reactive.
Purification
BHLHA15 antibody is affinity chromatography purified via peptide column.
Immunogène
BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15. The immunogen is located within amino acids 60 - 110 of BHLHA15.
BHLHA15
Reactivité: Humain, Souris
WB, FACS
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
BHLHA15 antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
BHLHA15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Antigène
BHLHA15
(Basic Helix-Loop-Helix Family, Member A15 (BHLHA15))
Autre désignation
BHLHA15
Sujet
BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).