alpha Tubulin anticorps (C-Term)

N° du produit ABIN6991938

Cet anticorps anti-alpha Tubulin est un anticorps Lapin Polyclonal détectant alpha Tubulin dans WB, ELISA, IF et IHC (p). Adapté pour Humain, Souris et Rat.

Aperçu rapide pour alpha Tubulin anticorps (C-Term) (ABIN6991938)

Antigène: alpha Tubulin (TUBA1)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp alpha Tubulin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-alpha Tubulin anticorps

Épitope: C-Term

Specificité: Alpha-Tubulin antibody is human, mouse and rat reactive.

Purification: alpha-Tubulin antibody is affinity chromatography purified via peptide column.

Immunogène: alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin

Isotype: IgG

Information d'application

Indications d'application: alpha-Tubulin antibody can be used for detection of alpha-Tubulin by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: alpha-Tubulin antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: alpha-Tubulin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: alpha-Tubulin

Sujet: Alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

Poids moléculaire: Predicted: 50 kDa

Observed: 54 kDa

ID gène: 7846

Pathways: Dynamique des Microtubules