Rubicon anticorps (N-Term)

N° du produit ABIN6992053

Cet anticorps Lapin Polyclonal détecte spécifiquement Rubicon dans WB, ELISA, IF et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Rubicon anticorps (N-Term) (ABIN6992053)

Antigène: Rubicon (KIAA0226) (KIAA0226)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Rubicon est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Rubicon anticorps

Épitope: AA 240-290, N-Term

Specificité: Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.

Purification: Rubicon antibody is affinity chromatography purified via peptide column.

Immunogène: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon. The immunogen is located within amino acids 240 - 290 of Rubicon.

Isotype: IgG

Information d'application

Indications d'application: Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rubicon antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: Rubicon antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Détails sur Rubicon

Antigène: Rubicon (KIAA0226) (KIAA0226)

Autre désignation: Rubicon

Sujet: Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).

Poids moléculaire: Predicted: 90, 101, 109 kDa

Observed: 91, 99 kDa

ID gène: 9711

NCBI Accession: XP_005269431

UniProt: Q92622

Pathways: Autophagy