LIMA1 anticorps (N-Term)

N° du produit ABIN6992089

Cet anticorps Lapin Polyclonal détecte spécifiquement LIMA1 dans WB, ELISA, IF et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour LIMA1 anticorps (N-Term) (ABIN6992089)

Antigène: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIMA1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-LIMA1 anticorps

Épitope: AA 230-280, N-Term

Specificité: LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist, this antibody will only detect the three largest isoforms.

Purification: LIMA1 antibody is affinity chromatography purified via peptide column.

Immunogène: LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1. The immunogen is located within amino acids 230 - 280 of LIMA1.

Isotype: IgG

Information d'application

Indications d'application: LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: LIMA1 antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: LIMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Détails sur LIMA1

Antigène: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Autre désignation: LIMA1

Sujet: The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).

Poids moléculaire: Predicted: 50, 66, 84 kDa

Observed: 65 kDa

ID gène: 51474

NCBI Accession: NP_001107018

UniProt: Q9UHB6