Reelin anticorps (AA 3143-3461)

N° du produit ABIN7435136

Cet anticorps anti-Reelin est un anticorps Lapin Polyclonal détectant Reelin dans WB, IHC, IP et ICC. Adapté pour Souris. Validé indépendamment pour une utilisation dans Immunohistochemistry.

Aperçu rapide pour Reelin anticorps (AA 3143-3461) (ABIN7435136)

Antigène: Reelin (RELN)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Reelin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-Reelin anticorps

Épitope: AA 3143-3461

Fonction: Polyclonal Antibody to Reelin (RELN)

Specificité: The antibody is a rabbit polyclonal antibody raised against RELN. It has been selected for its ability to recognize RELN in immunohistochemical staining and western blotting.

Réactivité croisée: Humain, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogène: Recombinant Reelin (RELN) corresdonding to Glu3143~Pro3461 (Accession # Q60841)

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 0.01-5 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Commentaires:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #104517 (Immunohistochemistry)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #104517

Date: 24.01.2024

Antigène: Reelin

Numéro du lot: A20230619938

Application validée: Immunohistochemistry

Contrôle positif:

Postnatal day 5 and adult (> 2 months) CD1 mouse brain fixed in 4% paraformaldehyde.

Contrôle négative:

One control slice for each experimental procedure processed omitting the primary antibody; overnight incubation in diluent solution only.

Conclusion:

Passed. The Reelin antibody antibody (AA 3143-3461) ABIN7435136 works in for IHC-P at 1:100 concentration with microwave antigen retrieval.

Validation Images

Reelin expression in the postnatal day 5 mouse cerebellum. EGL = external granular layer of the cerebellar cortex; IGL = internal granular layer of the cerebellar cortex;PCL/ML = Purkinje cell/molecular layers of the cerebellar cortex; WM = white matter. Staining is mainly observed in the processes of the pre-migratory granule cells. Original magnifications: A=10x; B= 20x

Protocole

Anticorps primaire: ABIN7435136

Anticorps secondaire: anti-rabbit secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific A11037, lot # 2439060)

Full Protocol:

• Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix of samples in the same fixative for an additional 2 h at RT
• Wash, dehydrate, and embed samples in paraffin wax
• Wash several times with 0.01 M PBS
• Cut brain with a microtome into 6 µm sections and mount on glass slides
• After paraffin removal sections were processed for microwave antigen retrieval for 10 minutes (95-100° C) in 10 mM sodium citrate buffer (pH 6.0). After 20 minutes of spontaneous cooling, sections were washed (distilled water, 2 times for 5 minutes each; followed by PBS for 5 minutes)
• Incubate sections for 1 h at RT in a humid chamber in PBS containing 1% ovalbumin (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites
• Incubate sections with primary rabbit anti-Reelin (antibodies-online, ABIN7435136, lot A20230619938) diluted 1:50 and 1:100 in PBS-BSA-PLL (Sigma, P1524) ON at RT in a humid chamber
• Wash sections 3x 5 min with 0.01 M PBS
• Incubate sections with anti-rabbit secondary antibody Alexa Fluor 594, 1:500, in 0.1M PBS (Thermo Fisher A11037, lot # 2439060) for 1 h at RT
• Wash sections 3x 5 min with 0.01 M PBS
• Specimens were mounted in Fluoroshield (Sigma, F6182, lot # MKCB0153V)
• Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 40x magnification

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Date de péremption: 24 months

Détails sur Reelin

Antigène: Reelin (RELN)

Autre désignation: RELN

Sujet: RL

UniProt: Q60841

Pathways: Synaptic Membrane