ORC1 anticorps

N° du produit ABIN7252684

L’anticorps anti-ORC1 Polyclonal Lapin est utilisé pour la détection de ORC1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour ELISA et IHC. L’anticorps a en outre été validé indépendamment.

Aperçu rapide pour ORC1 anticorps (ABIN7252684)

Antigène: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ORC1 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC)

Détail du produit anti-ORC1 anticorps

Attributs du produit: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogène: Fusion protein of human ORC1

Isotype: IgG

Information d'application

Indications d'application: IHC 1:50-1:300, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #104637 (Immunohistochemistry)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #104637

Date: 18.03.2026

Antigène: Origin Recognition Complex, Subunit 1 (ORC1)

Numéro du lot: DW04006249610

Application validée: Immunohistochemistry

Contrôle positif:

CD1 mouse brain fixed in 4% paraformaldehyde.

Contrôle négative:

Omission of primary/secondary antibody.

Conclusion:

The antibody works in IHC-P and IHC (fro) at concentrations from 1:50-1:200. Microwave pretreatment is strongly suggested.

Validation Images

Origin Recognition Complex, Subunit 1 (ORC1L) immunoreactive cells in the cerebral cortex and hippocampus of P5 and adult CD1 mice. IHC-P. Magnification: 63x.

Protocole

Anticorps primaire: ABIN7252683

Anticorps secondaire: anti-rabbit Alexa Fluor 488 (Invitrogen A11008, lot # 3112790)

Full Protocol:

• IHC (p)
  • PROTOCOL
    • Sample analyzed: P5 and adult (9 months old) CD1 mouse brain
    • Paraffin-embedded slices (7µm thick) were deparaffinized and rehydrated through a graded series of alcohols and distilled water (dH2O)
    • Sections were blocked in ovalbumin 1% 1 hour at room temperature
    • Antigen retrieval was performed in all the sections by microwave treatment (1 minute at 750W + 1 minute at 250W in Citrate buffer pH 6)
    • 3x5 minutes washing in 0.01M PBS
    • Sections were incubated with the primary antibody at the following dilutions of 1:50/1:100/1:200, overnight at room temperature (primary antibody was diluted in PLL/BSA/PBS diluent)
    • 3x5 minutes washing in 0.01M PBS
    • Incubation with the anti-rabbit secondary antibody 1:400 in PLL/BSA/PBS diluent, 1 hour at room temperature.
    • 3x5 minutes washing in 0.01M PBS
    • Nuclear counterstaining was performed with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) 1µg/mL, 2 minutes
    • 2x5 minutes washing in 0.01M PBS
    • Specimens were then mounted in Fluoroshield mounting medium (Sigma)
    • Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 63x magnification
• IHC (fro)
  • PROTOCOL
    • Sample analyzed: old (22 months old) CD1 mouse brain
    • Samples were fixed in paraformaldehyde and cryoprotected with a series of sucrose solutions in 0.01M PBS at increasing concentrations (10%, 20%, 30%), then embedded with OCT compound in isopentane previously cooled in liquid nitrogen
    • Frozen slices (10µm thick) were washed 3x5 minutes in 0.01M PBS
    • Sections were blocked in ovalbumin 1% 1 hour at room temperature
    • Antigen retrieval was performed in all the sections by microwave treatment (1 minute at 750W + 1 minute at 250W in Citrate buffer pH 6)
    • 3x5 minutes washing in 0.01M PBS
    • Sections were incubated with the primary antibody at the following dilutions of 1:50/1:100/1:200, overnight at room temperature (primary antibody was diluted in PLL/BSA/PBS diluent)
    • 3x5 minutes washing in 0.01M PBS
    • Incubation with the anti-rabbit secondary antibody 1:400 in PLL/BSA/PBS diluent, 1 hour at room temperature.
    • 3x5 minutes washing in 0.01M PBS
    • Nuclear counterstaining was performed with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) 1µg/mL, 2 minutes
    • 2x5 minutes washing in 0.01M PBS
    • Specimens were then mounted in Fluoroshield mounting medium (Sigma)
    • Slides were observed under a Leica DM 6000B fluorescence microscope

Notes:

• For both IHC-P and IHC (fro) some slides were processed without microwave treatment to evaluate the actual necessity of antigen retrieval.
• To exclude any nonspecific signal due to the secondary antibody, one control slice was processed for each experimental procedure, omitting the primary antibody.

Stockage

Format: Liquid

Concentration: 1.14 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze / thaw cycles.

Détails sur ORC1

Antigène: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Autre désignation: ORC1

Sujet: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.ORC1 (Origin Recognition Complex Subunit 1) is a Protein Coding gene. Diseases associated with ORC1 include Meier-Gorlin Syndrome 1 and Microtia. Among its related pathways are E2F mediated regulation of DNA replication and CDK-mediated phosphorylation and removal of Cdc6. GO annotations related to this gene include chromatin binding. An important paralog of this gene is CDC6.

UniProt: Q13415

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA