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Histone 3 anticorps (H3K9me2)

Name: Histone 3 anticorps (H3K9me2)
SKU: ABIN703886
Availability: InStock

L’anticorps anti-Histone 3 Polyclonal Lapin est utilisé pour la détection de Histone 3 dans des échantillons de Humain. Il a été validé pour FACS, IHC (p), IF (cc), IF (p) et IHC (fro).

Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-Histone H3 (Di Methyl K9) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (Histone 3 anticorps (H3K9me2))

IHC (p)

HeLa cells were fixed with 70% ice-cold methanol overnight at 4℃, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. (Histone 3 anticorps (H3K9me2))

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Histone H3 (Di Methyl K9) Polyclonal Antibody, Unconjugated (ABIN703886) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37 °C and DAPI staining (Histone 3 anticorps (H3K9me2))

3 images

N° du produit ABIN703886

357,70 €

Plus frais de livraison 40,00 € et TVA

100 μL

Destination: France

Envoi sous 8 à 12 jours ouvrables

Aperçu rapide pour Histone 3 anticorps (H3K9me2) (ABIN703886)

Antigène

Voir toutes Histone 3 (H3) Anticorps

Histone 3 (H3) (Histone H3 (H3))

Reactivité

1822
927
782
48
37
36
33
31
25
24
23
19
8
6
6
5
5
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Humain

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp Histone 3 est non-conjugé

Application

1456
697
521
364
356
349
252
209
203
179
157
157
124
68
48
42
37
26
25
19
19
7
7
7
4
3
2
2
1
1
1
1
1
1
1

Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product H3 Anticorps primaire

Épitope
70

59

56

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49

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37

34

32

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27

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23

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19

17

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10

10
H3K9me2

Fonction

Histone H3 (Di Methyl K9) Antibody

Réactivité croisée

Humain, Rat

Homologie

Mouse,Cow,Pig,Rabbit,Fruit Fly

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic metylpeptide derived from human Histone H3 around metylation site of Lys9

Isotype

IgG
anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivité: Humain, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN Hôte: Lapin Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivité: Humain WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Hôte: Lapin Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivité: Humain, Saccharomyces cerevisiae WB, IF, IHC, ChIP, ICC, DB, ChIP-seq, CUT&Tag Hôte: Lapin Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivité: Humain, Souris WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag Hôte: Lapin Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivité: Humain, Souris WB, IF, ChIP, DB, ChIP-seq Hôte: Souris Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 Reactivité: Humain WB, IF, IHC, ChIP, IP, ChIP-seq Hôte: Lapin Polyclonal unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivité: Humain WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivité: Humain, Souris, Saccharomyces cerevisiae WB, IF, IHC, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN Hôte: Lapin Polyclonal unconjugated

anti-Histone H3 (H3) (H3R17me) antibody
H3 Reactivité: Humain WB, IF, IP Hôte: Lapin Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivité: Humain WB, IF, IHC, ChIP, IP, ChIP-seq Hôte: Lapin Polyclonal unconjugated

Indications d'application

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Antigène

Histone 3 (H3) (Histone H3 (H3))

Autre désignation

Histone H3

Sujet

Synonyms: H3F3, H3.3A, Histone H3.3, H3F3A, PP781
Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

ID gène

3020

UniProt

P84243