AQP2 anticorps (Intracellular)

N° du produit ABIN7042935

Cet anticorps anti-AQP2 est un anticorps Lapin Polyclonal détectant AQP2 dans WB, IHC et IC. Adapté pour Rat.

Aperçu rapide pour AQP2 anticorps (Intracellular) (ABIN7042935)

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AQP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunochromatography (IC)

Détail du produit anti-AQP2 anticorps

Épitope: AA 254-271, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Aquaporin 2 Channel

Specificité: Intracellular, C-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse - identical, human,sheep - 17,18 amino acid residues identical

Attributs du produit: Anti-Aquaporin 2 Antibody (ABIN7042935, ABIN7045196 and ABIN7045197) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize the AQP2 channel from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RQSVELHSPQSLPRGSKA, corresponding to amino acid residues 254-271 of rat AQP2

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Commentaires:

Cited Application: IP|IHC|ICC

Negative Control: (ABIN7234759)

Blocking Peptide: (ABIN7234759)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur AQP2

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Autre désignation: AQP2

Sujet: AQP2, AQP-CD, WCH-CD,Aquaporin 2 (AQP2) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP0 to AQP12). Little is known about the function of the two newest members, AQP11 and AQP12.The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP2 together with AQP1, AQP4 and AQP5 belongs to the first group.1The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1AQP2 expression is largely confined to the kidney, particularly in the renal collecting duct where it performs a key role in water absorption and urine concentration. In fact, mutations in the AQP2 gene produce hereditary nephrogenic diabetes insipidus, a disorder that results in the excretion of large volumes of urine.2Under normal conditions, water homeostasis in the kidney is regulated through the anti-diuretic hormone vasopressin. Vasopressin is secreted from the pituitary gland and transported to the kidney through the blood where it binds to its receptor that is mainly expressed in cells of the collecting duct. The activated vasopressin receptor induces an increase in intracellular cAMP and subsequent PKA activation, which phosphorylates AQP2. This phosphorylation causes the translocation of AQP2 channels from intracellular vesicles to the cell membrane where it markedly increases water permeability.1,3,4

Alternative names: AQP2, AQP2

ID gène: 25386

NCBI Accession: NM_000486

UniProt: P34080

Pathways: Response to Water Deprivation