Calnexin anticorps (Intracellular)

N° du produit ABIN7043021

L’anticorps Lapin Polyclonal anti-Calnexin a été validé pour WB, IHC et IF. Il convient pour détecter Calnexin dans des échantillons de Rat.

Aperçu rapide pour Calnexin anticorps (Intracellular) (ABIN7043021)

Antigène: Calnexin (CANX)

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Calnexin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-Calnexin anticorps

Épitope: AA 572-587, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Calnexin

Specificité: Intracellular, C-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,human - identical

Attributs du produit: Anti-Calnexin Antibody (ABIN7043021, ABIN7044212 and ABIN7044213) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Canx from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KAEEDEILNRSPRNRK, corresponding to amino acid residues 572-587 of rat Canx

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400-1:600

Commentaires:

Negative Control: (ABIN7235025)

Blocking Peptide: (ABIN7235025)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur Calnexin

Antigène: Calnexin (CANX)

Autre désignation: CANX

Sujet: Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90,Calnexin is a type I transmembrane endoplasmic reticulum chaperone protein involved in the folding and assembly of numerous proteins. Calnexin is comprised of 537 amino acids with a large luminal domain consisting of 462 amino acids. Calnexin does not possess N-linked glycosylation sites and is localized to the endoplasmic reticulum by a -RKPRRE motif at its carboxyl terminus. Calnexin is one of the few ER membrane proteins that can be phosphorylated in a GTP-dependent manner1.Calnexin interacts with a number of ER membrane channels. It interacts transiently with wild-type Shaker K+ channel in the ER but fails to associate with an unglycosylated Shaker mutant that makes active, cell surface channels. This suggest that glycosylation of Shaker protein is required for interaction with calnexin but calnexin is not required for the proper assembly and folding of Shaker channels2.Coexpression of calnexin with the voltage-gated potassium channel KV1.2α was found to produce a substantial dose-dependent increase in cell surface KV1.2α channels in transfected mammalian COS-1 cells. In contrast, calnexin coexpression showed no effect on trafficking of intracellularly retained KV1.1 or KV1.6α subunits3.Calnexin has been implicated in the pathophysiology of cystic fibrosis disease (CF). Wild type and ΔF508 mutant cystic-fibrosis conductance regulator (CFTR) associate with calnexin in a Chinese hamster ovary (CHO) cell model. Although both wild-type and ΔF508 CFTRs are present in complexes with calnexin, only wild-type CFTR is able to escape this association and exit the ER. This suggests that calnexin retains misfolded proteins in the ER and contributes to the mislocalization of mutant CFTRs.

Alternative names: Calnexin, Canx, Calx, CNX, Major histocompatibility complex class I antigen-binding protein p88, p90, IP90

ID gène: 29144

NCBI Accession: NM_001024649

UniProt: P35565

Pathways: Signalisation MAPK, Thyroid Hormone Synthesis