EFCAB4B anticorps (Intracellular)

N° du produit ABIN7043114

L’anticorps Lapin Polyclonal anti-EFCAB4B a été validé pour WB. Il convient pour détecter EFCAB4B dans des échantillons de Humain.

Aperçu rapide pour EFCAB4B anticorps (Intracellular) (ABIN7043114)

Antigène: EFCAB4B (EF-Hand Calcium Binding Domain 4B (EFCAB4B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EFCAB4B est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-EFCAB4B anticorps

Épitope: AA 245-258, Intracellular

Fonction: A Rabbit Polyclonal Antibody to CRAC Channel Regulator 2A

Specificité: Intracellular

Réactivité croisée: Humain, Rat

Homologie: Rat - 13,14 amino acid residues identical

Attributs du produit: Anti-CRACR2A (EFCAB4B) Antibody is directed against an epitope of the human CRAC channel regulator 2A. Anti-CRACR2A (EFCAB4B) Antibody (ABIN7043114, ABIN7044115 and ABIN7044116) can be used in western blot analysis. It has been designed to recognize CRAC channel regulator 2A from rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KSEKEQFLLKDTER, corresponding to amino acid residues 245-258 of human CRACR2A

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Commentaires:

Negative Control: (ABIN7235259)

Blocking Peptide: (ABIN7235259)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur EFCAB4B

Antigène: EFCAB4B (EF-Hand Calcium Binding Domain 4B (EFCAB4B))

Autre désignation: CRACR2A

Sujet: CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B,Calcium is a vital ion for the functioning of many intracellular processes. When Ca2+ stored in the endoplasmic reticulum is depleted, there is an activation of store-operated channels that results in the influx of Ca2+ from outside the cell. In non-excitable cells and especially in immune cells, most of this influx is carried out by calcium release activated calcium channels also known as "CRACs"1.Mediation of signals from the ER to CRACs and activation of the CRAC channel is conducted by two modulators that physically cluster with the CRAC channel known as Orai1 and STIM1. STIM1 is a single transmembrane segment protein that has a long C-terminal cytoplasmic region and an N-terminus which detects ER Ca2+ depletion. Orai1 is a macromolecular complex with linkers of varying lengths between the ER and plasma membranes.Novel findings suggest the existence of a complex comprised of Orai1, STIM1 and additional proteins. One of these proteins is an EF-hand protein named CRACR2A which regulates Orai-STIM interaction. An EF-hand is a structural domain found in Ca2+ binding proteins consisting of two alpha helices linked by a short loop region.CRACR2A interacts with the cytoplasmic regions of both Orai1 and STIM1 thus creating a tertiary complex. Under high concentrations of Ca2+, CRACR2A caused a dissociation of Orai1 and STIM1 and a mutated CRACR2A was found, in one study, to cause elevated Ca2+ levels that caused cell death in T cells2.Further research is required to fully determine the activation and regulation mechanisms of CRACs and this can be performed by better understanding CRACR2A and its binding proteins.

Alternative names: CRACR2A (EFCAB4B), CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B

ID gène: 84766

NCBI Accession: NM_032680

UniProt: Q9BSW2