EPH Receptor A3 anticorps (Extracellular)

N° du produit ABIN7043123

L’anticorps Lapin Polyclonal anti-EPH Receptor A3 a été validé pour WB, IHC, FACS, IF et LCI. Il convient pour détecter EPH Receptor A3 dans des échantillons de Humain.

Aperçu rapide pour EPH Receptor A3 anticorps (Extracellular) (ABIN7043123)

Antigène: EPH Receptor A3 (EPHA3)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EPH Receptor A3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Détail du produit anti-EPH Receptor A3 anticorps

Épitope: AA 488-500, Extracellular

Fonction: A Rabbit Polyclonal Antibody to EphA3 Receptor

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat,mouse - identical

Attributs du produit: Anti-EphA3 (extracellular) Antibody (ABIN7043123, ABIN7044245 and ABIN7044246) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize EphA3 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RARGTNVTISSLK, corresponding to amino acid residues 488-500 of human EphA3

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235282)

Blocking Peptide: (ABIN7235282)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur EPH Receptor A3

Antigène: EPH Receptor A3 (EPHA3)

Autre désignation: EPHA3

Sujet: Ephrin type-A receptor 3, EPH-like kinase 4, EK4, Tyrosine-protein kinase TYRO4,Eph receptors are the largest family of receptor tyrosine kinases (RTKs). Based on sequence similarity and the type of ephrin ligands that they bind, they are classified into A and B sub-classes. EphA receptors bind ephrin-A ligand while EphB bind ephrin-B ligand.Eph receptors play an important role during embryonic development and are involved in several processes including cell adhesion, cell migration, and tissue boundary formation. Eph receptors are typically expressed at low levels in normal adult tissues1,2.EphA3 (Ephrin type-A receptor 3) structure includes a ligand-binding domain (LBD) at the N-terminus followed by a cysteine-rich region and two fibronectin III repeats. The receptor-binding domain of the ephrins is connected through a short linker segment to a GPI-anchor or a transmembrane segment3.EphA3 was first identified as a surface antigen on pre-B lymphoblastic leukemia cells. High EphA3 levels have been detected in a number of hematological cancers and solid tumors such as lung cancers, melanomas, gastric carcinoma, leukemia, in B and T cell malignancies and in glioblastoma multiforme. In addition, high levels of EphA3 are implicated in lymph node metastasis, advanced stages of colorectal cancer, and prostate cancer1,3,4.

Alternative names: EphA3, Ephrin type-A receptor 3, EPH-like kinase 4, EK4, Tyrosine-protein kinase TYRO4

ID gène: 2042

NCBI Accession: NM_005233

UniProt: P29320

Pathways: Signalisation RTK, Regulation of Cell Size