EPH Receptor B1 anticorps (Extracellular)

N° du produit ABIN7043126

L’anticorps Lapin Polyclonal anti-EPH Receptor B1 a été validé pour WB, IF, FACS, IC et LCI. Il convient pour détecter EPH Receptor B1 dans des échantillons de Souris.

Aperçu rapide pour EPH Receptor B1 anticorps (Extracellular) (ABIN7043126)

Antigène: EPH Receptor B1 (EPHB1)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EPH Receptor B1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Détail du produit anti-EPH Receptor B1 anticorps

Épitope: AA 485-497, Extracellular

Fonction: A Rabbit Polyclonal Antibody to EphB1 Receptor

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat,human - identical

Attributs du produit: Anti-EphB1 (extracellular) Antibody is directed against an extracellular epitope of mouse EphB1. Anti-EphB1 (extracellular) Antibody (ABIN7043126, ABIN7044251 and ABIN7044252) can be used in western blot, immunocytochemistry and indirect flow cytometry applications. The antibody is suitable for live cell imaging. It has been designed to recognize EphB1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSQTNTARIDGLR, corresponding to amino acid residues 485-497 of mouse EphB1

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:800

Commentaires:

Negative Control: (ABIN7235294)

Blocking Peptide: (ABIN7235294)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur EPH Receptor B1

Antigène: EPH Receptor B1 (EPHB1)

Autre désignation: EPHB1

Sujet: Ephrin type-B receptor 1, Eph receptor B1, Tyrosine-protein kinase receptor EPH-2,Eph receptors are the largest family of receptor tyrosine kinases (RTKs). EphA receptors (EphA1-10) bind ephrinA ligands which are GPI-linked proteins and EphB receptors (EphB1-6) bind ephrinB ligands which are membrane protein with one transmembrane domain1,2. Within each subfamily, interactions between receptor and ligand are promiscuous. In addition, Eph receptors can also bind ephrins from the other class2. Forward and reverse signaling through Eph receptors is a unique characteristic to this RTK since ephrins are physically linked to the plasma membrane3.Structurally, Eph receptors contain an extracellular ligand-binding domain, a transmembrane domain and an intracellular C-terminal domain responsible for intracellular signaling1. Forward Eph receptor signaling involves autophosphorylation of the receptor via a tyrosine kinase domain, as well as phosphorylation of other proteins. Known effectors of the forward signaling include Src kinase and Ras/Rho GTPases2. Much less is known about the reverse signaling mediated by Eph receptors. Besides from acting independently, Eph receptors can also signal in concert with other receptors. For example, Eph receptors cooperate with FGF receptor, NMDA ligand-gated ion channel and chemokine G-protein coupled receptor2.Biological activities attributed to the Eph receptor-ephrin signaling module include establishing neuronal connections, mediating neuronal plasticity and repair following neuronal injury2,4. Eph receptors may also have a role in the immune system5.Eph receptors are expressed in the developing nervous system, and in the adult brain. It is also detected in the pancreas, intestine, bone and lymphocytes. In cancer cells, Eph receptors and ephrins are overexpressed2,6,7. They are also implicated in neurodegenerative disorders like Alzheimer's disease1.

Alternative names: EphB1, Ephrin type-B receptor 1, Eph receptor B1, Tyrosine-protein kinase receptor EPH-2

ID gène: 270190

NCBI Accession: NM_004441

UniProt: Q8CBF3

Pathways: Signalisation RTK