GRIN2B anticorps (Extracellular)

N° du produit ABIN7043230

Cet anticorps Lapin Polyclonal détecte spécifiquement GRIN2B dans WB, IHC, IP, IF, IC et LCI. Il présente une réactivité envers Rat.

Aperçu rapide pour GRIN2B anticorps (Extracellular) (ABIN7043230)

Antigène: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRIN2B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Détail du produit anti-GRIN2B anticorps

Épitope: Extracellular

Fonction: A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B)

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Human,mouse - identical

Attributs du produit: Anti-NMDAR2B (GluN2B) (extracellular) Antibody (ABIN7043230, ABIN7044326 and ABIN7044327) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize GluN2B from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDA receptor 2B

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:600

Commentaires:

Cited Application: IP|IHC|ICC|IFC

Negative Control: (ABIN7235630)

Blocking Peptide: (ABIN7235630)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur GRIN2B

Antigène: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Autre désignation: GRIN2B

Sujet: NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B,The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's disease.NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Alternative names: NMDAR2B (GluN2B), NMDA receptor 2B, Ionotropic glutamate receptor subunit epsilon2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B

ID gène: 24410

NCBI Accession: NM_000834

UniProt: Q00960

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity