HCRTR1 anticorps (AA 256-269)

N° du produit ABIN7043285

L’anticorps Lapin Polyclonal anti-HCRTR1 a été validé pour WB, IHC, IF et IC. Il convient pour détecter HCRTR1 dans des échantillons de Rat.

Aperçu rapide pour HCRTR1 anticorps (AA 256-269) (ABIN7043285)

Antigène: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HCRTR1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Détail du produit anti-HCRTR1 anticorps

Épitope: AA 256-269

Fonction: A Rabbit Polyclonal Antibody to Orexin Receptor 1

Specificité: 3rd intracellular loop

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse - identical, human - 9,14 amino acid residues identical

Attributs du produit: Anti-Orexin Receptor 1 Antibody (ABIN7043285, ABIN7044883 and ABIN7044884) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX1R from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50-1:100

Application Dilutions Western blot wb: 1:200-1:500

Commentaires:

Cited Application: IHC

Negative Control: (ABIN7235802)

Blocking Peptide: (ABIN7235802)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur HCRTR1

Antigène: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Autre désignation: HCRTR1

Sujet: OX1R, Hypocretin receptor type 1, HCRTR1,Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Alternative names: Orexin Receptor 1, OX1R, Hypocretin receptor type 1, HCRTR1

ID gène: 25593

NCBI Accession: NM_001525

UniProt: P56718

Pathways: Feeding Behaviour