Nerve Growth Factor anticorps (AA 84-104)

N° du produit ABIN7043555

L’anticorps Cobaye Polyclonal anti-Nerve Growth Factor a été validé pour WB, IHC et IF. Il convient pour détecter Nerve Growth Factor dans des échantillons de Rat.

Aperçu rapide pour Nerve Growth Factor anticorps (AA 84-104) (ABIN7043555)

Antigène: Nerve Growth Factor (NGF)

Reactivité: Rat

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp Nerve Growth Factor est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-Nerve Growth Factor anticorps

Épitope: AA 84-104

Fonction: A Guinea Pig Polyclonal Antibody to proNGF

Specificité: Pro-domain of the NGF protein

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.

Homologie: Mouse - identical, human - 16,21 amino acid residues identical

Attributs du produit: Guinea pig Anti-proNGF Antibody is directed against rat proNGF. Guinea pig Anti-proNGF Antibody can be used in western blot and immunohistochemistry applications. Anti-proNGF Antibody raised in guinea pig recognizes the same epitope as Anti-proNGF Antibody (ABIN7043556, ABIN7044751 and ABIN7044752) raised in rabbit. The antibody specifically reacts with proNGF and does not crossreact with proBDNF, pro or mature NGF. It has been designed to recognize proNGF from mouse, rat and human samples. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc. \nRelated products for neutralizing proNGF activity: \nMouse NGF/proNGF Neutralizing Antibody can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7236601)

Blocking Peptide: (ABIN7236601)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur Nerve Growth Factor

Antigène: Nerve Growth Factor (NGF)

Autre désignation: NGF

Sujet: Nerve growth factor precursor,Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins.It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8

Alternative names: proNGF, Nerve growth factor precursor

ID gène: 310738

NCBI Accession: NM_002506

UniProt: P25427

Pathways: Regulation of Cell Size