Nerve Growth Factor anticorps (Pro-Domain)

N° du produit ABIN7043555

L’anticorps anti-Nerve Growth Factor Polyclonal Cobaye est utilisé pour la détection de Nerve Growth Factor dans des échantillons de Rat. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour Nerve Growth Factor anticorps (Pro-Domain) (ABIN7043555)

Antigène: Nerve Growth Factor (NGF)

Reactivité: Rat

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp Nerve Growth Factor est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-Nerve Growth Factor anticorps

Épitope: AA 84-104, Pro-Domain

Fonction: A Guinea Pig Polyclonal Antibody to proNGF

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.

Homologie: Mouse - identical, human - 16,21 amino acid residues identical

Attributs du produit: Guinea pig Anti-proNGF Antibody is directed against rat proNGF. Guinea pig Anti-proNGF Antibody can be used in western blot and immunohistochemistry applications. Anti-proNGF Antibody raised in guinea pig recognizes the same epitope as Anti-proNGF Antibody (ABIN7043556, ABIN7044751 and ABIN7044752) raised in rabbit. The antibody specifically reacts with proNGF and does not crossreact with proBDNF, pro or mature NGF. It has been designed to recognize proNGF from mouse, rat and human samples. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc. \nRelated products for neutralizing proNGF activity: \nMouse NGF/proNGF Neutralizing Antibody can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7236601)

Blocking Peptide: (ABIN7236601)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Nerve Growth Factor

Antigène: Nerve Growth Factor (NGF)

Autre désignation: NGF

Sujet:

Synonyms: Nerve growth factor precursor

Description: Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways. It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4 Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins.It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8

ID gène: 310738

NCBI Accession: NM_002506

UniProt: P25427

Pathways: Regulation of Cell Size