SLC32A1 anticorps (Cytosolic)

N° du produit ABIN7043718

Cet anticorps anti-SLC32A1 Polyclonal Cobaye (ABIN7043718) détecte spécifiquement SLC32A1 dans WB et IHC. L’anticorps est réactif avec des échantillons de Rat.

Aperçu rapide pour SLC32A1 anticorps (Cytosolic) (ABIN7043718)

Antigène: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Reactivité: Rat

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC32A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-SLC32A1 anticorps

Épitope: AA 106-120, Cytosolic

Fonction: A Guinea Pig Polyclonal Antibody to Vesicular GABA Transporter

Specificité: Cytoplasmic, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,human - identical

Attributs du produit: Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody , raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize VGAT from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-Vesicular GABA Transporter (VGAT) Antibody (ABIN7043719, ABIN7044467 and ABIN7044468) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200

Commentaires:

Cited Application: ICC

Negative Control: (ABIN7237075)

Blocking Peptide: (ABIN7237075)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur SLC32A1

Antigène: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Autre désignation: SLC32A1

Sujet: Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, rGVAT, SLC32A1,The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)1.VGAT is responsible for the uptake of GABA and glycine in exchange of protons, and their release from nerve terminals1.The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail2. In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown3.VGAT is expressed on synaptic vesicles of GABAergic and glycynergic neurons4-6. The transporter is also detected in the pituitary gland and in the pancreas7,8.

Alternative names: Vesicular GABA Transporter (VGAT), Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, rGVAT, SLC32A1

ID gène: 83612

NCBI Accession: NM_080552

UniProt: O35458

Pathways: Proton Transport