SLC32A1 anticorps (N-Term)

N° du produit ABIN2855225

Détail du produit anti-SLC32A1 anticorps

Antigène: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Épitope: N-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC32A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Multiplex Immunohistochemistry (mIHC)

Attributs du produit: Rabbit Polyclonal antibody to VGAT (solute carrier family 32 (GABA vesicular transporter), member 1)
VGAT antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the N-terminus region of human VGAT. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Commentaires:

Positive Control: U87-MG , mouse brain

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104339 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104339

Date: 20.04.2021

Antigène: SLC32A1

Numéro du lot: 42838

Application validée: Multiplex Immunohistochemistry

Contrôle positif:

FFPE normal human cortex

Contrôle négative:

Unlabeled control (FFPE normal human cortex)

Conclusion:

Passed. The anti-SLC32A1 antibody ABIN2855225 produces punctate staining in close approximation with dendritic processes. This pattern is consistent with presynaptic staining, which is the expected site of SLC31A1 immunofluorescence.

Validation Images

FFPE normal human cortex tissue section labeled with anti-SLC32A1 antibody ABIN2855225 (green; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and SYN1 were labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-SYN1 antibody ABIN5542390 (blue; bound to fluorophore AF488).

Protocole

Anticorps primaire: ABIN2855225

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-SLC32A1 antibody ABIN2855225 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.

Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of SLC32A1 was observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur SLC32A1

Antigène: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Autre désignation: VGAT (SLC32A1 Produits)

Synonymes: anticorps VGAT, anticorps VIAAT, anticorps vGAT, anticorps Ci-vGAT, anticorps slc32a1, anticorps viaat, anticorps MGC68938, anticorps vgat, anticorps xVIAAT, anticorps R75019, anticorps Viaat, anticorps CG8394, anticorps CG8394.2, anticorps Dmel\\CG8394, anticorps Vgat, anticorps zgc:158324, anticorps solute carrier family 32 member 1, anticorps vesicular GABA transporter, anticorps solute carrier family 32 member 1 S homeolog, anticorps vesicular inhibitory amino acid transporter, anticorps Vesicular inhibitory amino acid transporter, anticorps solute carrier family 32 (GABA vesicular transporter), member 1, anticorps Vesicular GABA Transporter, anticorps SLC32A1, anticorps vgat, anticorps Bm1_20950, anticorps slc32a1.S, anticorps slc32a1, anticorps CpipJ_CPIJ002704, anticorps viaat, anticorps Slc32a1, anticorps VGAT, anticorps Tsp_04599

Sujet: The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.

Cellular Localization: Cytoplasmic vesicle membrane, Multi-pass membrane protein

Poids moléculaire: 57 kDa

ID gène: 140679

Pathways: Proton Transport