Recombinant Ly6g anticorps

N° du produit ABIN7072246

Cet anticorps Lapin Chimeric détecte spécifiquement Ly6g dans FACS et Dep. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour Recombinant Ly6g anticorps (ABIN7072246)

Antigène: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp Ly6g est non-conjugé

Application: Flow Cytometry (FACS), Depletion (Dep)

Clone: 1A8

Détail du produit anti-Ly6g anticorps

Fonction: Anti-Ly6G [1A8], Rabbit IgG, kappa

Specificité: Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells

Isotype: IgG kappa

Information d'application

Indications d'application: Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion < a href="http://www.ncbi.nlm.nih.gov/pubmed/17884993" target="_blank"> in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) < a href="http://absoluteantibody.com/ab/anti-f480-cia3-1-recombinant-version/" target="_blank"> (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022, PMID: 36359801).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Ly6g

Antigène: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Autre désignation: Ly6G

Sujet: LGR-1 , y-6G, GR1, Lymphocyte antigen 6G

UniProt: P35461