Recombinant CD16 anticorps

N° du produit ABIN7072393

Cet anticorps Lapin Chimeric détecte spécifiquement CD16 dans FACS, IP, IHC, BR, Dep et SPR. Il présente une réactivité envers Humain, Macaque rhésus, Cynomolgus, Babouin, Capuchin Monkey, Chimpanzé, Macaca nemestrina, Marmoset, Saguinus oedipus, Sooty mangabey (Cercocebus atys) et Squirrel Monkey.

Aperçu rapide pour Recombinant CD16 anticorps (ABIN7072393)

Antigène: CD16

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Macaque rhésus, Cynomolgus, Babouin, Capuchin Monkey, Chimpanzé, Macaca nemestrina, Marmoset, Saguinus oedipus, Sooty mangabey (Cercocebus atys), Squirrel Monkey

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp CD16 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Blocking Reagent (BR), Depletion (Dep), Surface Plasmon Resonance (SPR)

Clone: 3G8

Détail du produit anti-CD16 anticorps

Fonction: Anti-Fc gamma receptor III [3G8], Rabbit IgG, kappa

Specificité: 3G8 binds to CD16 at an epitope which is not the IgG-binding site. This antibody cannot distinguish between CD16a transmembrane isoform found on the surface of NK cells and macrophages (which can trigger the cytolytic response), and the GPI-linked CD16b isoform present on neutrophils or soluble CD16 found in the plasma. CD16 is the low affinity IgG receptor III and binds to aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis and ADCC (antibody-dependent cell-mediated cytotoxicity).

Réactivité croisée: Babouin, Capuchin Monkey, Chimpanzé, Cynomolgus, Macaca nemestrina, Marmoset, Macaque rhésus, Saguinus oedipus, Sooty mangabey (Cercocebus atys), Squirrel Monkey

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: 3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.

Isotype: IgG kappa

Information d'application

Indications d'application: 3G8 antibody was used to construct a recombinant bispecific scFv (bsscFv) along with the mAb clone F3.3 anti-HLA class II antibody (connected by a (Gly4Ser)4 linker)which was expressed in SF21 insect cells. This bsscFv allows NK cells to target malignant B lymphoid cells and mediates effector cell lysis (ASCC) of the malignant cells (also lysis of primary leukaemia samples). 3G8 is also able to block neutrophil phagocytosis and stimulates NK cell proliferation, and can be used in FC, SPR, IP and IHC studies.

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD16

Antigène: CD16

Autre désignation: Fc gamma receptor III

Sujet: CD16, low affinity IgG receptor III, FcγRIII, low affinity FcγRIII

UniProt: Q9ULV2

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process