Recombinant Hemoglobin anticorps

N° du produit ABIN7072452

Cet anticorps anti-Hemoglobin est un anticorps Lapin Chimeric détectant Hemoglobin dans WB et ELISA. Adapté pour Souris.

Aperçu rapide pour Recombinant Hemoglobin anticorps (ABIN7072452)

Antigène: Hemoglobin

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp Hemoglobin est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 2A1

Détail du produit anti-Hemoglobin anticorps

Fonction: Anti-Haemoglobin [2A1], Rabbit IgG, kappa

Specificité: 2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogène: Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend's Adjuvant.

Isotype: IgG kappa

Information d'application

Indications d'application: 2A1 has been used to bind to mHb in Western Blots and has been found to increase the secretion of TNF-alpha from endothelial cells when co-incubated with Hb, suggesting that 2A1 induces pro-inflammatory events (Bhatnagar 2011).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Hemoglobin

Antigène: Hemoglobin

Autre désignation: Haemoglobin

Sujet: Hemoglobin, Hb

UniProt: P01942