Recombinant CCRL2 anticorps (AA 1-24)

N° du produit ABIN7072483

Cet anticorps Lapin Chimeric détecte spécifiquement CCRL2 dans WB, ELISA, IHC, FACS, ICC, Func et IP. Il présente une réactivité envers Souris.

Aperçu rapide pour Recombinant CCRL2 anticorps (AA 1-24) (ABIN7072483)

Antigène: CCRL2 (Chemokine (C-C Motif) Receptor-Like 2 (CCRL2))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp CCRL2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Functional Studies (Func), Immunoprecipitation (IP)

Clone: BZ5B8

Détail du produit anti-CCRL2 anticorps

Épitope: AA 1-24

Fonction: Anti-CCRL2 [BZ5B8], Rabbit IgG, kappa

Specificité: This antibody is specific for murine CCRL2 (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2 presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2 antibody binding.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising Wistar Furth rats with the mCCRL2 peptide/KLH conjugate. The immunizing amino-terminal mCCRL2 peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody has been used in various FACS analyses, for instance, to confirm that it is only specific to mCCRL2-hemagglutinin (HA)/L1.2 transfectants and that peritoneal macrophages treated with LPS, TNFα, IFN-γ, or poly:IC up-regulate mCCRL2 protein expression (Zabel et al., 2008). Of note, this antibody binding to mouse peritoneal mast cells could be inhibited by chemerin, a protein ligand for signaling receptor CMKLR1 (Zabel et al., 2008). This anti-CCRL2 antibody can be used in wide variety of assays to study leukocytes and immune response. For example, this antibody has been used to examine the expression, regulation, and function of atypical chemerin receptor CCRL2 on endothelial cells (Monnier et al., 2012) and to delineate how the chemoattractant chemerin suppresses melanoma by recruiting natural killer cell antitumor defenses (Pachynski et al., 2012).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CCRL2

Antigène: CCRL2 (Chemokine (C-C Motif) Receptor-Like 2 (CCRL2))

Autre désignation: CCRL2

Sujet: CCR11, C-C chemokine receptor-like 2, Chemokine receptor CCR11, CKRX, CRAM-A, CRAM-B, G-protein coupled beta chemokine receptor, L-CCR, Lipopolysaccharide-inducible C-C chemokine receptor

UniProt: O35457