Recombinant TIMD4 anticorps

N° du produit ABIN7072499

L’anticorps Lapin Chimeric anti-TIMD4 a été validé pour ELISA, FACS, IP et in vivo. Il convient pour détecter TIMD4 dans des échantillons de Souris.

Aperçu rapide pour Recombinant TIMD4 anticorps (ABIN7072499)

Antigène: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp TIMD4 est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), In vivo Studies (in vivo)

Clone: 5G3

Détail du produit anti-TIMD4 anticorps

Fonction: Anti-Tim-4 [5G3], Rabbit IgG, kappa

Specificité: This antibody is specific for murine TIM-4. TIM-4, unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen-presenting cells such as lymphoid and myeloid dendritic cells and macrophages. TIM-4 has been shown to interact with TIM-1, thereby suggesting a role of TIM-4 in the balance of Th1/Th2 regulation. Additionally, TIM-4 has been shown to be a receptor for phosphatidylserine and may play a role in the engulfment of apoptotic cells.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: This antibody was generated by immunizing a female Lewis rat with Tim-4-Ig fusion protein.

Isotype: IgG kappa

Information d'application

Indications d'application: The original version of this antibody (rat IgG1) specifically bound murine Tim-4 protein, as shown in ELISA and FC assays. The antibody was also used to immunoprecipitate Tim-4 protein from lysates of CHO-Tim-4 as part of WB assays (Rodriguez-Manzanet et al., 2008, PMID: 18354194). This antibody can block apoptotic body (AB) recognition by CD11b⁺ peritoneal macrophages and partially inhibit AB uptake by peritoneal B-1 cells (Rodriguez-Manzanet et al., 2010, PMID: 20368430). treatment with this antibody alone did not significantly affect tumor burden or survival, but it enhanced the protective effect of anti-PD-1 blockade. The combined treatment resulted in an increase in total CD8⁺ and CD8⁺ CD39⁺ T cells in the peritoneal cavity. RNA sequencing of sorted Tim-4⁺ macrophages revealed genes differentially expressed between animals treated with anti-PD-1 alone and those treated with both anti-PD-1 and this antibody, indicating that it alters the gene expression profile of Tim-4⁺ macrophages. Notably, it reduced the expression of genes associated with immunosuppression (Ptgs2, Tgfb2, and Tdo2) while increasing the expression of genes associated with macrophage activation (Stab1 and Mrc1). This antibody was used as part of single-cell RNA sequencing and T-cell receptor sequencing analyses. This antibody was used for FC staining in the form of flow sorting, co-culture experiments, and flow cytometric analysis to investigate various aspects of Tim-4 function, including its role in the sequestration of cytotoxic CD8⁺ T cells, the impairment of CD8⁺ T cell proliferation by cavity-resident macrophages, and the assessment of Tim-4-mediated suppression of CD8⁺ T cells both in vitro and in vivo. For example, it was used for FC staining in the form of annexin V staining to detect phosphatidylserine (PS) exposure on the surface of CD8⁺ T cells (Chow et al., 2021, PMID: 34115989).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur TIMD4

Antigène: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Autre désignation: Tim-4

Sujet: TIMD4, SMUCKLER, SMUCKLER, TIM4, Spleen, mucin-containing, knockout of lymphotoxin protein, T-cell immunoglobulin and mucin domain containing 4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4, TIMD-4

UniProt: Q6U7R4

Pathways: Cancer Immune Checkpoints