Recombinant CD40 anticorps

N° du produit ABIN7072518

Cet anticorps anti-CD40 est un anticorps Rat Monoclonal détectant CD40 dans FACS, IHC et IP. Adapté pour Souris.

Aperçu rapide pour Recombinant CD40 anticorps (ABIN7072518)

Antigène: CD40

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 3-23

Détail du produit anti-CD40 anticorps

Fonction: Anti-CD40 [3/23], Rat IgG2a, kappa

Specificité: This antibody is specific for murine CD40, a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising Lou rats with murine CD40-hgamma1-Fc according to the protocol by Hasbold et al. (Eur J Immunol. 1994)

Isotype: IgG2a kappa

Information d'application

Indications d'application: This antibody has been used in various FACS analyses, for instance, to determine the immunoregulatory properties of primary colonic epithelial cells (Telega et al., 2000), to validate if eicosapentaenoic acid induces prolonged survival of cardiac allografts and generates regulatory T cells (Iwami et al., 2009), to characterise Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors (Zhang et al., 2006), to delineate the mechanisms for the activation of dendritic cells and induction of T cell responses by HPV 16 L1/E7 chimeric virus-like particles (Freyschmidt et al., 2004), and to investigate the role of epigenetic modification and immunomodulation in a murine prostate cancer model (Sulek et al., 2017). In a study by White et al. (J Immunol. 2011), the parental anti-mouse CD40 3/23 (rat IgG2a) mAb was first reformatted to mouse IgG1 (m1) and IgG2a (m2a) versions. The new variants were then utilised in immunohistochemistry, SPR, and in vitro phagocytosis assays and assessments for B cell proliferation, activation, and survival to confirm that interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 amAb (White et al. 2011).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD40

Antigène: CD40

Autre désignation: CD40

Sujet: TNFRSF5, B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, MGC9013, T-cell differentiation antigen, Tumor necrosis factor receptor superfamily member 5, tumor necrosis factor receptor superfamily, member 5

UniProt: P27512

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints