Recombinant CD35 anticorps

N° du produit ABIN7072538

L’anticorps Souris Monoclonal anti-CD35 a été validé pour WB, IF et ELISA. Il convient pour détecter CD35 dans des échantillons de Humain.

Aperçu rapide pour Recombinant CD35 anticorps (ABIN7072538)

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD35 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Clone: E11

Détail du produit anti-CD35 anticorps

Fonction: Anti-CR1 [E11], Mouse IgG1, kappa

Specificité: E11 is reactive to neutrophilic granulocytes and eosinophils, monocytes, macrophages, lymphocytes, erythrocytes, dendritic reticulum cells, tonsil tissue section, spleen tissue section, lymph node tissue section, gut tissue section, kidney podocytes and kidney tissue sections, chronic lymphocytic leukemia, acute myelomonocytic leukemia, acute monocyte leukemia, B cell lines (JY, MST, BR17), EBV-infected B cell cultures, myeloid cell lines (U937, HL60, THP 1-0).

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: BALB/c mice were immunised with cells from acute monocytic leukemia patients.

Isotype: IgG1 kappa

Information d'application

Indications d'application: E11 fluorescence staining of normal blood cells was detectable on 90 % of monocytes, 95 % of neutrophils, and 14 %-24 % of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD35

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Autre désignation: CR1

Sujet: CD35, Type 1 complement receptor, C3b/C4b receptor

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity