Recombinant Transferrin Receptor anticorps

N° du produit ABIN7072543

Cet anticorps Rat Monoclonal détecte spécifiquement Transferrin Receptor dans FACS et Dep. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour Recombinant Transferrin Receptor anticorps (ABIN7072543)

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin Receptor est non-conjugé

Application: Flow Cytometry (FACS), Depletion (Dep)

Clone: RI7 217-1-3

Détail du produit anti-Transferrin Receptor anticorps

Fonction: Anti-Transferrin receptor [RI7 217.1.3], Rat IgG2a, kappa

Specificité:

The transferrin receptor is necessary for cellular iron uptake by the process of receptor-mediated endocytosis. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis.

Application Notes original Clone: This antibody was generated and used in a flow cytometry assay to identify murine hematopoietic progenitor cells expressing the transferrin receptor (Lesley et al., 1984, PMID: 6319033). The original format of this antibody was tested and found to moderately reduce cell growth. A Fab version of this antibody was generated, its binding was tested, and it was compared to its full antibody counterpart. In contrast to the original antibody format, the Fab fragment did not cause receptor down-regulation or accelerated degradation. Both the Fab fragment and the full antibody were successfully used in an IF staining assay (Lesley et al., 1989, PMID: 2653853). The original fotmat of this antibody was demonstrated to undergo receptor-mediated transcytosis across the blood-brain barrier (BBB), as confirmed by capillary depletion studies, and to effectively transport into the mouse brain in vivo (Lee et al., 2000, PMID: 10688622). Injection of this antibody into mice resulted in CD71⁺ erythroid splenocyte cell depletion (Torow et al., 2015, PMID: 26195040).

Purification: Protein A affinity purified

Immunogène: The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Rat

OriginalFormat: IgG2a

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Transferrin Receptor

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: Transferrin receptor

Sujet: CD71, Transferrin receptor protein 1, TR, , TfR1, Trfr, R17 217.1.3, RI7-217

Pathways: Transition Metal Ion Homeostasis